High-speed camera captures signals traveling through nerve cells.

Diff-CUP operates similarly to Wang’s other CUP systems, which have been shown capable of recording video at 70 trillion frames per second and capturing images of laser pulses as they travel at the speed of light.

Diff-CUP takes the same high-speed camera technology found in the other CUP systems and combines it with a device called a Mach–Zehnder interferometer. The interferometer images objects and materials by first splitting a beam of laser light in two, passing only one of the split beams through an object, and then recombining the beams. Because light waves are affected by the objects they pass through, with different materials affecting them in varying ways, the beam passing through the material being imaged will have its waves set out of sync with the waves of the other beam. When the beams are recombined, the out-of-sync waves interfere with each other (hence “interferometer”) in patterns that reveal information about the object being imaged.

Though you cannot see an electrical pulse traveling through a nerve cell with your own eyes, or even a conventional light microscope, this type of interferometry can detect it. (Incidentally, this same basic technique is used by LIGO to detect gravitational waves.) So, the Mach–Zehnder interferometer allows the imaging of these pulses, and the CUP camera captures the images at incredibly high frame rates.

“Seeing nerve signals is fundamental to our scientific understanding but has not yet been achieved owing to the lack of speed and sensitivity provided by existing imaging methods,” Wang says.

Wang’s research team also captured photos of the propagation of electromagnetic pulses (EMP), which, in some materials, can travel at nearly the speed of light. In this case, they passed the electromagnetic pulses through a crystal of lithium niobate, a salt that has unique optical and electrical properties. Despite the extremely high speed at which an EMP passes through this material, the camera was able to clearly image it.

“Imaging propagating signals in peripheral nerves is the first step,” Wang says. “It’d be important to image live traffic in a central nervous system, which would shed light on how the brain works.”

The paper describing their findings, titled “Ultrafast and hypersensitive phase imaging of propagating internodal current flows in myelinated axons and electromagnetic pulses in dielectrics,” appeared in the journal Nature Communications on September 6. Co-authors are Yide Zhang, postdoctoral scholar research associate in medical engineering; Binglin Shen, visitor from Shenzhen University; Tong Wu, visitor from Nanjing University of Aeronautics and Astronautics; Jerry Zhao, former graduate student of the USC–Caltech MD-PhD program; Joseph C. Jing, formerly of Caltech and currently at Cepton; Peng Wang, senior postdoctoral scholar research associate in medical engineering; Kanomi Sasaki-Capela, former research technician at Caltech; William G. Dunphy, Grace C. Steele Professor of Biology; David Garrett, graduate student in medical engineering; Konstantin Maslov, former staff scientist at Caltech; and Weiwei Wang of University of Texas Southwestern Medical Center.

Funding for the research was provided by the National Institutes of Health.